AneuSure

For rapid detection of chromosomal aneuploidies for chromosomes 21, 18, 13, X and Y using QF-PCR technology.

AneuSure Kit consists of 26 markers. The STR markers are distributed across autosomal chromosomes 21, 18 and 13 and sex chromosomes X & Y. The segmental duplication 7X marker is included in the kit for the differentiation of X chromosome monosomy from homozygosity (i.e. it quantifies the chromosome X for more accurate detection of Turner syndrome. AneuSure performance has been validated with extensive testing using Applied BiosystemsTM 3500/3500xL and 3130/3130xl platforms for detection and analysis on more than 1000 samples.

Features

  • Easy to use mix
  • Multiplex analysis of 26 loci in one reaction
  • ~ 90 min thermal clycling time
  • Accurate detection of Turner syndrome
  • Applicable to a variety of DNA sources – amniotic fluid (AF), chorionic villus (CVS), fetal tissue, etc.

For Research Use only. Not for use in diagnostic procedures

NameSizeCat. No.
AneuSure (Free Sample)10 RXNGT-11101-10
AneuSure25 RXNGT-11101-25
AneuSure50 RXNGT-11101-50
AneuSure100 RXNGT-11101-100

Quick ProtocolREQUEST FOR DOCUMENT
User Instruction ManualREQUEST FOR DOCUMENT
Quality Control DNADownload
Down Syndrome (+21) ProfileDownload
Edward Syndrome (+18) ProfileDownload
Patau Syndrome (+13) ProfileDownload
Triploidy 69 ProfileDownload
Turner Syndrome (XO) ProfileDownload
Klinefelter Syndrome (XXY) ProfileDownload
Triple X Syndrome (XXX) ProfileDownload
Control DNADownload
PCR MixDownload
Primer MixDownload
WaterDownload

Certificate of Analysis

To find a Certificate of Analysis, enter a lot number in the search field below. If you need assistance, please send us an email to support[at]genetek.de or call us under +49 (0)30-6392 7050 in Berlin.

  

What is chromosomal abnormalities?

Chromosomal numerical abnormality is termed aneuploidy. In most cases they are resulted due to nondisjunction either at first or second miotic cell division. However, very rarely they are the result of first or later stages of mitotic cell division of the embryo. The later one may result in mosaicism.

Chromosome specific markers

Simultaneous analysis of 26 loci circumvent the use of extra kits or markers, though GENETEK has chromosome specific STR markers for chromosome 21, 18 and 13. They can be ordered separately to avoid being charged unnecessarily.

Why it is important to diagnose chromosomal abnormalities?

Most cases of aneuploidy are detrimental and either stops the formation of a viable fetus or causes abortion in the early stages of fetal development. It is the most common chromosomal aberration with clinical importance in humans.

It is of high frequency in embryos and exists in 3 to 4% of recognized pregnancies and 1 in 160 live births. The most common chromosomal aneuploidies at birth are related to either of X, Y, 21, 18 and 13 chromosomes. The three later ones and monosomy of X (Turner Syndrome) are of more clinical importance.

What is QF- PCR?

The conventional method for the detection of aneuploidy in the fetuses is karyotyping. This method is usually time consuming and laborious.

QF-PCR is a Quantitative Fluorescence-Polymerase Chain Reaction. It is a reliable alternative to karyotyping for the detection of chromosomal aneuploidy. This method is a novel, fast and cost-effective molecular technique for prenatal diagnosis of chromosomes aneuploidies.

It is a PCR- based method using fluorescently labeled primers. DNA extracted from amniotic fluid, chorionic villus samples and blood can be used as the genomic material in this method. In a multiplex assay, several specific STR markers for each chromosome are amplified which their peaks represent the number of chromosomes.

References:

Umberto Nicolini, Faustina Lalatta, Federica Natacci, Cristina Curcio, The-Hung Bui; The introduction of QF-PCR in prenatal diagnosis of fetal aneuploidies: time for reconsideration, Human Reproduction Update, Volume 10, Issue 6, 1 December 2004, Pages 541–548, https://doi.org/10.1093/humupd/dmh046